MD5 Verification Methods

The directory that typically needs MD5 verification is the Rawdata folder (usually named 00.Rawdata).

Rawdata# usually the 00.Rawdata folder

your_fastq.gz

md5.md5# text file containing MD5 values

md5check.exe# MD5 verification tool for Windows

md5check.apple# MD5 verification tool for macOS (md5check_apple)

Open the md5check.exe file located in the Rawdata folder to begin the check.
If all samples show "Pass" or "OK", it indicates that the check was successful.

Tip

If the md5check.exe file is not present, please click here to download it and save it to the Rawdata folder before running it.

Locate the Rawdata folder (the folder containing the md5.md5 file) and check whether a file named md5check_apple exists inside it.
Open Terminal: press Command + Space to open Spotlight, type Terminal, and press Enter.
Type cd followed by a space, then drag the Rawdata folder into the Terminal window and press Enter.
If md5check_apple is not present in the Rawdata directory, run the following command to download it. Skip this step if the file already exists.

curl -o md5check_apple https://azenta-transfer-doc.oss-cn-hangzhou.aliyuncs.com/rel/md5/md5check-darwin-arm64

Once the file is downloaded (or if it was already present), run the following two commands in order:

chmod +x md5check_apple

./md5check_apple

Open Terminal
Use the cd command to navigate to the directory containing the MD5 verification files
Run the following command:
```
 md5sum -c md5.md5
```

What if the MD5 verification is inconsistent?

Please contact our after-sales support team for assistance.

#MD5 Verification Methods